Sensory Dendrite Length by Anchoring Dendritic Tips during Cell Migration
نویسندگان
چکیده
Constructing the dex-1(ns42); dyf-7(ns117) strain The dex-1(ns42); dyf-7(ns117) strain was generated using the genetic balancer hT2[qIs48], a homozygous lethal translocation marked with myo-2pro:GFP, pes10pro:GFP, and ges-1pro:GFP (Miskowski et al., 2001), to derive a strain of genotype hT2[qIs48]/+ I; hT2[qIs48]/dex-1(ns42) III; dyf-7(ns117) X. When cultivated at 20°C, this strain showed 100% segregation of the qIs48 GFP markers, implying that dex1(ns42) III; dyf-7(ns117) X progeny were inviable. By cultivating this strain at 25°C, we recovered animals lacking the qIs48 GFP markers that we inferred to be dex-1(ns42) III; dyf-7(ns117) X, and this genotype was confirmed by DNA sequencing.
منابع مشابه
DEX-1 and DYF-7 Establish Sensory Dendrite Length by Anchoring Dendritic Tips during Cell Migration
Cells are devices whose structures delimit function. For example, in the nervous system, neuronal and glial shapes dictate paths of information flow. To understand how cells acquire their shapes, we examined the formation of a sense organ in C. elegans. Using time-lapse imaging, we found that sensory dendrites form by stationary anchoring of dendritic tips during cell-body migration. A genetic ...
متن کاملIntrinsic mechanisms to define neuron class-specific dendrite arbor morphology.
The class-specific transcription factors Knot and Cut act during dendrite arbor development to define the characteristic dendrite branching pattern of the Drosophila class IV dendritic arborisation sensory neurons. Knot mediates dendrite arbor outgrowth and branching via a microtubule-based program that includes upregulation of the microtubule severing protein Spastin. On the other hand, Cut pr...
متن کاملAssisted morphogenesis: glial control of dendrite shapes.
Neurons display a myriad of dendritic architectures, reflecting their diverse roles in information processing and transduction in the nervous system. Recent findings suggest that neuronal signals may not account for all aspects of dendrite morphogenesis. Observations from C. elegans and other organisms suggest that glial cells can affect dendrite length and guidance, as well as localization and...
متن کاملLithium Dendrite Growth Control Using Local Temperature Variation
We have quantified lithium dendrite growth in an optically accessible symmetric Limetal cell, charged under imposed temperatures on the electrode surface. We have found that the dendrite length measure is reduced up to 43% upon increasing anodic temperature of about 50 0 C. We have deduced that imposing higher temperature on the electrode surface will augment the reduction rate relative to dend...
متن کاملNak Regulates Localization of Clathrin Sites in Higher-Order Dendrites to Promote Local Dendrite Growth
VIDEO ABSTRACT During development, dendrites arborize in a field several hundred folds of their soma size, a process regulated by intrinsic transcription program and cell adhesion molecule (CAM)-mediated interaction. However, underlying cellular machineries that govern distal higher-order dendrite extension remain largely unknown. Here, we show that Nak, a clathrin adaptor-associated kinase, pr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2009